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ALDHl与干细胞和肿瘤干细胞的分选鉴定 被引量：4

Application of ALDHl in isolation and identification of stem cells and cancer stem cells
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摘要 乙醛脱氢酶1(aldehyde dehydrogenase 1,ALDH1)是一种可以将醛类氧化为乙酸的胞质溶酶,其在胚胎干细胞和成体干细胞中表达增高,对维持干细胞(stem cell,SC)的"干性"中起着重要作用。近年发现,ALDH1在肿瘤干细胞(cancerstem cell,CSC)中表达也升高。基于ALDH1活性的Aldefluo分析法已成功分选和鉴定造血干细胞、乳腺干细胞和神经干细胞等多种SC,也已成功分选和鉴定白血病干细胞、乳腺癌干细胞、肺癌干细胞、膀胱癌干细胞和头颈部肿瘤干细胞等多种CSC。免疫组化原位染色能有效检测ALDH1活性,从而也可鉴定SC和CSC,目前正在研究将此方法应用于临床评估肿瘤患者的预后。ALDH1已成为分选和鉴定SC和CSC的通用标志物之一,是SC研究的得力工具。
作者 董华英 吴诚义
机构地区 重庆医科大学附属第一医院内分泌外科
出处 《中国肿瘤生物治疗杂志》 CAS CSCD 北大核心 2011年第6期682-685,共4页 Chinese Journal of Cancer Biotherapy
基金 重庆市卫生局医学科研项目(No.06-2-028)~~
关键词 乙醛脱氢酶1 干细胞 肿瘤干细胞 Aldefluor分析法 分选 鉴定
分类号 Q279 [生物学—细胞生物学] R730.2 [医药卫生—肿瘤]
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参考文献8

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同被引文献52

  • 1杨毅,吴星娆,李岚,李娅.恶性纤维组织细胞瘤的诊治进展[J].实用癌症杂志,2006,21(4):443-444. 被引量：12
  • 2杨复宾,周珉,陈方.肠系膜恶性纤维组织细胞瘤一例[J].放射学实践,2007,22(1):27-27. 被引量：9
  • 3千建荣,李建民,谢飞彬,杨志平,李昕.骨的恶性纤维组织细胞瘤诊治分析[J].中国矫形外科杂志,2007,15(5):351-354. 被引量：9
  • 4CHEN Yong-bing,YAN Mao-lin,GONG Jian-ping,XIA Ren-pin,LIU Li-xin,LI Ning,LU Shi-chun,ZHANG Jing-guang,ZENG Dao-bing,XIE Jian-guo,YANG Jia-yin,YAN Lü-nan.Establishment of hepatocellular carcinoma multidrug resistant monoclone cell line HepG2/mdr1[J].Chinese Medical Journal,2007(8):703-707. 被引量：16
  • 5Bonnet D, Dick JE. Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell [J]. Nat ned, 1997, 3(7) : 730-737.
  • 6Chiba T, Kita K, Zheng YW, et al. Side population purified from hepatocellular carcinoma cells harbors cancer stem cell-like properties [ J ]. Hepatology, 2006, 44 ( 1 ) : 240-251.
  • 7O' Brien CA, Pollett A, Gallinger S, et al. A human colon cancer cell capable of initiating tumour growth in immunodeficient mice [J]. Nature, 2007, 445(7123) : 106-110.
  • 8Patrawala L, Calhoun T, Schneider-Broussard R, et al. Side population is enriched in tumorigenic, stem-like cancer ceils, whereas ABCG2^+ and ABCG2^+ cancer cells are similarly tumorigenic [J]. Cancer Res, 2005, 65 (14): 6207-6219.
  • 9Dontu G, Abdallah WM, Foley JM, et al. In vitro propagation and transcriptional profiling of human mammary stem/progenitor ceils [J]. Genes Dev, 2003, 17(10) : 1253-1270.
  • 10Mimeault M, Hauke R, Mehta PP, et al. Recent advances in cancer stem/progenitor cell research: Therapeutic implications for overcoming resistance to the most aggressive cancers [ J ]. J Cell Mol Med, 2007, 11(5) : 981-1011.

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中国肿瘤生物治疗杂志
2011年 第6期

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