细脚拟青霉甘露醇含量的测定

Determination of Mannitol in Paecilomyces tenuipes Mycelium and Its Culture Medium

采用比色法在波长412nm处测定细脚拟青霉菌丝体及固体培养物中甘露醇含量。考察提取溶剂、提取温度、提取时间对甘露醇提取率的影响,得到甘露醇最佳提取条件,以蒸馏水为提取溶剂,菌丝体在60℃时提取60min,固体培养物在10 0℃时提取60m in;对测定方法进行回收率、精密度、稳定性实验,菌丝体中甘露醇回收率为99.94%,相对标准偏差为0.74%,精密度实验相对标准偏差为0.80%,60min内光密度值稳定,固体培养物中甘露醇回收率99.79%,相对标准偏差为1.17%,精密度实验相对标准偏差为1.00%,60min内光密度值稳定。

The mannitol content of the mycelium of Paecilomyces tenuipes and its culture medium was analyzed colorimetrically at 412 nm.The effects of extraction solvent,temperature and time on extraction yield of mannitol were investigated to optimize the extraction conditions.The best results of mannitol extraction were achieved by using distilled water to extract Paecilomyces tenuipes mycelium at 60 ℃ and extract its culture medium at 100 ℃ for 60 min both.Under these conditions,the recovery rate of mannitol from Paecilomyces tenuipes mycelium was 99.94% with a RSD of 0.74%,the precision RSD was 0.80% and a good stability of the OD value at 412 nm was found within 60 min.In the case of mannitol extraction from the culture medium,the recovery,RSD and precision RSD were 99.79%,1.17%,and 1.00%,respectively,and the OD value at 412 nm also showed a good stability within 60 min.