摘要
目的研究经济简便的可用于延胡索药材的含量测定的方法。方法采用乙醚回流提取,以石油醚-乙酸乙酯-无水乙醇(2:1.7:01)为展开剂,碘为显色剂,用薄层层析-紫外分光光度法测定延胡索中延胡索乙素的含量。结果延胡索供试品与对照品在相应位置上显相同颜色的斑点,延胡索乙素在浓度0-25mg/L的范围内呈良好线性关系,回归方程:A=00106+0.016C,r=09995。加样回收率为991%,IKSD=098%。结论实验结果表明,薄层层析一紫外分光光度法测定延胡索中延胡索乙素的含量,此法操作简单,重现性好,结果稳定。
Objective To study simple can used for the method of the content measurement that yanhusuo. Method Use ethoxy ethane to extract,outspread agent was petroleum ether-ethyl acetate-ethano](2:1.7:0.1) and developer was/2 steam.Determination of Dl-Tetrahydropamatine in yanhusuo by TLC - UV.Results Contrast sample and examine sample display same colour spot at same position.Linearity range was 0-25 mg/L, r=0.9995, The average recovery is 99.1%, RSD=0.98%.Conclusion The method is simple and the results are accurate,This method can be used for quality control of yanhusuo.
作者
徐丹丹
偶志红
曲婷丽
刘锐玲
XU Dan-dan, OU Zhi-hong, QU Ting-li, LIU Rui-ling (Pharmaceutical College, Shanxi Medical University, Taiyuan 030001,China)
出处
《医学信息》
2011年第23期199-200,共2页
Journal of Medical Information