葛根素对肾性高血压大鼠apelin-12、AngⅡ及NO含量与血压的影响

Effects of puerarin on apelin-12,angiotensin II,NO and blood pressure in renovascular hypertensive rat

目的:观察葛根素对肾性高血压大鼠血压的影响,并通过观察apelin-12、血管紧张素Ⅱ(Ang II)和一氧化氮(NO)含量的变化,初步探讨葛根素的降压机制。方法:Sprague-Dawley(SD)大鼠65只,雄性,随机抽取8只作为假手术组,其余采用两肾一夹法造成肾性高血压大鼠模型。将造模成功大鼠随机分成5组:葛根素高、中、低剂量组、卡托普利组和模型组。给药6周,每2周测1次血压。给药6周后取血,取肾脏。ELISA法测肾脏、血清中apelin-12含量,放射免疫法测肾脏、血浆中Ang II含量,硝酸还原酶法测血清中NO含量。结果:葛根素具有降压作用,并且随着剂量的升高血压下降越明显。葛根素高、中剂量组血清中apelin-12含量显著下降(P<0.01),葛根素低剂量组则下降不明显,但仍有统计学意义(P<0.05)。肾脏中apelin-12含量随着葛根素剂量的升高而逐渐降低。葛根素高、中剂量均能显著降低血浆中Ang II含量(P<0.01),低剂量葛根素则影响不显著(P>0.05)。葛根素高、中剂量均能显著升高血清中NO含量(P<0.01),低剂量葛根素则影响不明显(P>0.05)。结论:葛根素具有降压作用,其机制可能与其改变大鼠体内apelin-12、AngⅡ和NO的含量及调节它们之间的平衡有关。

AIM： To investigate the effects of puerarin on blood pressure in renovascular hypertensive rats,and to measure puerarin-induced changes of apelin-12,angiotensin II（Ang II） and nitric oxide（NO）,the factors related to development of hypertension.METHODS： Sixty-five male Sprague-Dawley rats were used,of which 8 rats were randomly selected as sham operation group,and the remaining were used to make two-kidney,one-clip model.The rats that met the criterion for Goldblatt hypertensive rat model were randomly allocated into 5 groups： high-,middle-and low-dose puerarin groups,captopril group,and model group.The drugs were administered for 6 weeks.Blood pressure was measured every 2 weeks.Six weeks after treatment,all rats were sacrificed under deep anesthesia.Blood and kidney samples were collected.The level of apelin-12 in serum and kidneys was detected by ELISA.The level of Ang II in plasma and kidneys was measured by radioimmunoassay.NO level in serum was examined by nitrate reductase assay.RESULTS： Puerarin had an antihypertensive effect in a dose-dependent manner.Marked decreases in the level of serum apelin-12 in high-and middle-dose puerarin groups were observed（P0.01）.Puerarin at low dose did not cause obvious change in the content of apelin but still reached significant level（P0.05）.As the dose of puerarin went up,the level of apelin-12 in the kidneys was gradually decreased.Puerarin at high and middle doses obviously reduced the level of AngII in plasma,while purarin at low dose did not produce any significant effects.Puerarin at high and middle doses markedly increased the level of NO in serum,but puerarin at low dose did not induce any significant changes.CONCLUSION： Puerarin has an antihypertensive effect,and its mechanism may be related to inducing the changes of apelin,Ang II and NO,and regulating the balance among those factors.