摘要
应力作用下软骨细胞代谢和功能的改变是导致软骨组织不断生长改建的主要原因。但目前,动态压力作用下调控软骨细胞生物学行为变化的分子机制尚不清楚。该研究借助于蛋白质组学技术,通过给藻酸盐三维培养的软骨细胞施加0.2 MPa、0.66 Hz动态压力持续作用4 h,比较加压组和未加压组蛋白表达的变化。结果10个蛋白点发生了变化:2个在加压培养后表达消失,2个在加压培养后出现表达,6个表达增强。进一步通过质谱分析和蛋白鉴定识别出6个有意义的蛋白质,分别是:proly14-hydroxylase alpha I、pyruvate kinase、L-lactate dehydrogenase A、proly1 4-hydroxylasesubunitβ、destrin isoform和αenolase。为今后深入研究这些差异蛋白在软骨细胞受压力刺激后代谢和功能变化的生物学作用奠定了基础。
Mechanical stresses are known to play important role in articular cartilage functions in vivo. However, the molecular events of chondrocytes in respose to dynamic stress are still not well understood so far. In the present study, rabbit articular cartilage cells cultured in alginate beads were exposed to 0.2 MPa, 0.66Hz cyclic loadings for 4 hours. The differently expressed proteins involved in chondrocytes cultured in alginate beads under dynamic stress loading and unloading were identified by using comparative proteomics technology, l0 differently expressed proteins were found. In the 10 proteins, there were 6 up-regulated ones, 2 disappeared ones and 2 new ap- pearance. Furthermore, by searching in protein and pertide databases, six meaningful proteins were identified, they were: prolyl 4-hydroxylase alpha I, pyruvate kinase, L-lactate dehydrogenase A, prolyl 4-hydroxylase subunit beta, destrin isoform and alpha enolase. Our research provided fundamental information on the differential expression of protein of chondrocytes cultured in alginate under dynamic stress. However, futher studies need to be done to better understand the molecular mechanisms of cartilage remodeling induced by dynamic stress loading.
出处
《中国细胞生物学学报》
CAS
CSCD
2011年第12期1350-1356,共7页
Chinese Journal of Cell Biology
基金
国家自然科学基金(No.30870594)
兰州大学中央高校基本科研业务费专项资金(No.LZUJBKY-2010-141)资助项目~~
关键词
软骨细胞
海藻酸盐
压力
差异蛋白质组学
chondrocytes
alginate
mechanical compression
comparative proteomics -