参黄冲剂对衰老患者NO和SOD含量及临床疗效的影响

Effect of Shenhuang Electuary on Senile Patients' NO and SOD Concentration and Clinical Therapeutic Effectiveness

目的:通过观察参黄冲剂对衰老患者血清NO和SOD含量等相关内环境指标影响与个体反应差异的关联性,评价参黄冲剂在延缓衰老方面的作用和临床疗效。方法:采用随机、单盲、对照研究方法,共选择60例符合条件的衰老患者纳入研究及统计分析。所有患者被随机分为参黄冲剂治疗组和对照治疗组,每组30人。参黄冲剂组进行常规治疗基础上加用参黄冲剂治疗(江苏省中医院协定处方),每次10g,每日3次。对照组进行常规系统治疗,并给予甲磺酸双氢麦角毒碱片,每次1mg,每日3次。各组均连续服药8周为1个疗程,每7天复诊一次。观察时间为1个疗程。观察治疗前后衰老临床症状及血清NO和SOD含量的变化和个体反应差异的关联性。结果:衰老患者血清NO含量,参黄冲剂组治疗前后具有统计学意义(P<0.05),对照组治疗前后无统计学意义(P>0.05),治疗后参黄冲剂组积分明显高于对照组,具有统计学意义(P<0.05);衰老患者血清SOD含量,两组治疗前后自身对照显示,参黄冲剂组治疗后SOD水平明显升高,差异具有统计学意义(P<0.05),对照组治疗前后差异无统计学意义(P>0.05);治疗后组间比较显示,参黄冲剂组明显高于对照组,差异具有显著意义。两组治疗后衰老患者临床疗效比较,参黄冲剂组30例,显效3例,有效21例,无效6例,总有效率80.0%;对照组30例,显效1例,有效12例,无效17例,总有效率43.3%;经统计学处理,两组间有效率有统计学意义(P<0.01)。参黄冲剂组治疗前后两种证型症状积分比较无统计学意义(P>0.05)。结论:参黄冲剂治疗老年肾虚患者,可有效改善临床衰老的临床症状,提高日常生活能力;参黄冲剂组治疗前后两种证型症状积分比较无统计学意义,证明参黄冲剂对不同证型衰老患者均有疗效;而且参黄冲剂还可以提高血清NO含量降低血清SOD含量,通过清除体内自由基,发挥抗氧化损伤保护作用,保护细胞免受损伤,对机体氧化和抗氧化平衡起重要作用,为进一步诠释中医辨证施治衰老的机理提供分子生物学的依据。

Objective ： To investigate the anti - aging effect and clinical therapeutic effectiveness of Shenhuang electuary through observing the relevance between Shenhuang electuary＇s effect on environmental indexes, such as NO and SOD concentration in senile patients＇ serum, and individual reactive differences. Methods： 60 cases of senile patients were choosen into the research and statistical analysis was made by the principle of random, single - blind, and control. All patients were randomly divided into two groups：Shenhuang electuary treatment group and control group, each group contains 30 cases. The treatment group were treated with conventional medical treatment plus Shenhuang electuary（ cipher prescrip- tion of The Traditional Chinese Medicine Hospital of Jiangsu Province） , 10g every time, tid. and the control group were treated with conventional medical treatment plus Co - dergocrine Mesylate Tablets, 1 mg every time, tid. Each group lasted continuous 8 weeks as one treatment,and one reture visit every 7days. Observation period was one treatment. Senile patients＇ clinical symptoms and relevance between serum NO and SOD concentration and individual reactive differences between pre and post treatment were observed. Results ： For aging patients, Shenhuang electuary treatment group had statistical significance in pre and post treatment（P 〈 0.05）, compared with the control group had no statistical significance （P 〉 0.05）. The score of Shenhuang electuary treatment group was higher significantly compared with the control group, showing statistical significant（ P 〈 0.05 ）. Serum SOD content of aging patient, the auto - control observation of two group showed ： NO content of Shenhuang electuary treatment group had higher significance after treatment, with statistical significance（ P 〈 0.05 ）, compared with the control group had no statistical siginificance in pre and post treatment（ P 〉 0.05 ） ; the comparison between groups after treatment showed：Shenhuang electuary treatment group was higher significantly compared with the control group, showing statistical significance （ P 〈 0.05 ）. The comparison between two groups on aging patients after treatment showed：30cases of Shenhuang electuary treatment group, 3 cases with noticeable effects, 21 cases improving and 6 cases with no effects, total effective rate was 80.0% ;30 cases of the control group ： 1 cases with noticeable effects, 12 cases improving and 17 cases with no effects, total effective rate was 43.3% ;there was a significant difference of the tolal response rate between the two groups, processed by statistics （P 〈 0.01 ）. The symptom score of Shen- huang electuary treatment group had no statistical siginificance in pre and post treatment（ P 〉 0.05 ）. Conclusion： Using Shenhuang electuary to treat senile patients with kidney deficiency can effectively improve clinical symptoms and living a- bility. The symptom score of Shenhuang electuary treatment group had no statistical siginificance in pre and post treatment, showing that Shenhuang electuary all had efficacy on different symptoms. Moreover Shenhuang electuary can improve serum NO concentration and decrease serum SOD content, by clearing free radical in vivo, play a role of antioxida- tion and protecting cells from injury, which is essential to balance between oxidation and antioxidation, it provides molecular biological evidence to further explain mechanism of treating senile patients by TCM differentiation.