金莲花中总黄酮的分光光度法测定研究

Determination of Total Flavonoids in Trollius Chinensis Bunge by Spectrophotometry

目的:建立分光光度法测定金莲花中总黄酮的方法。方法:以芦丁为对照品,采用AlCl3显色分光光度法,在最大吸收波长420 nm处,对金莲花中总黄酮进行定量测定。结果:测得金莲花中总黄酮为93.34 mg/g,方法的相对标准偏差(RSD)为0.55%(n=6),加标回收率在95.91%～101.42%之间。结论:选用芦丁为对照品,通过AlCl3显色,分光光度法测定金莲花中总黄酮,方法简单,准确度较高。

Objective： To establish a spectropbotometry to determine total flavonoids in TroUius chinensis Bunge. Methods： With Rutin being taken as reference substance, Total flavonids in Trollius chinensis Bunge were determined by spectrophotometry, through AlCl3 Colorimetry at 420 nm. Results： The content of flavonoids was 93.34 mg/g in Trollius chinensis Bunge, the RSD was 0.55 % （ n = 6） , and recoveries ranged from 95.91% to 101.42 %. Conclusion： To determine content of total flavonoids in Trollius chinensis Bunge with spectrophotometry through AlCl3 Colorimetry by standard Rutin is a simple method with higher accuracy.