电针足三里穴对兔单肺通气所致氧化应激反应的影响

Effect of Electroacupuncture of "Zusanli"(ST 36) on Oxidative Stress Rseponse by One Lung Ventilation

目的:观察电针足三里对兔单肺通气期间氧化应激途径及肺损伤程度的影响。方法:健康成年新西兰兔39只,随机分为3组(n=13):电针非穴位组(EN组)、电针穴位组(EP组)、对照组(C组)。麻醉后气管切开,插入气管导管,双肺通气30min后,行右肺单肺通气,3 h后重新恢复双肺通气。EN、EP两组于单肺通气开始时实施电针刺激,EN组取双侧足三里穴旁1cm处,EP组取双侧足三里穴处。实验结束后处死动物,测定肺组织丙二醛(MDA)、谷胱甘肽过氧化酶(GSH-PX)和超氧化物歧化酶(SOD)含量,观察肺组织病理形态学,计算肺组织湿/干重比(W/D)。结果:EP组与其他两组比较,MDA明显降低(P<0.05),且GSH-PX、SOD升高(P<0.05);肺间质及肺泡水肿和岀血较少,仅见少量中性粒细胞浸润,且W/D明显较低(P<0.05)。结论:电针足三里可以通过抑制氧化应激反应来减少单肺通气引起的肺损伤。

Objective ： To observe the effects of electroacupuncture of ＂Zusanli＂ （ ST 36） on oxidative stress rseponse and lung injury degree during one lung ventilation. Methods ：39 healthy New Zealand rabbits were randomized into three groups （n = 13 ） ：electroacupuncture at no point group （EN）, electroacupuncture at Zusanli points （EP）and control group （ C ）. Tracheostomy was performed following with anesthesia, and endotracheal tube was inserted. Two lungs were ventilated for 30 min, followed by single right lung ventilation for 3 h, by two lung ventilation, electroacupuncture were administered in group EN and group EP when one lung ventilation began. Electroacupuncture was stimulated at somewhere 1 cm nearby Zusanli in group EN, at Zusanli points in group EP. Animals were executed at end, determined the MDA, GSH - PX, SOD level in the lung tissues , observed the pathomorphoiogy of the lung, calculated the wet/dry of the lung. Results：Compared with other groups, MDA was reduced significantly and GSH - PX and SOD levels were increased in group EP（P 〈0.05）. The edema, hemorrhage at lung intersitium and alveolus were lower, and less neutrophil infihrated,W/D was lowed significantly in group EP（ P 〈 0. 05 ）. Conclusions： Electroacupuncture may reduce lung injury induced by one lung ventilation by suppressing oxidative stress response.