黄芩苷对中波紫外线辐射后小鼠皮肤氧化应激及DNA损伤的影响

Effects of Baicalin on UVB-mediated Oxidative Stress and DNA Damages in Mice Skins

目的观察黄芩苷对中波紫外线(UVB)辐射后小鼠皮肤氧化应激及DNA损伤的影响。方法将黄芩苷外搽于Balb/c小鼠皮肤,检测180mJ/cm2UVB辐射后24h小鼠皮肤厚度及过氧化氢和CPDs产量。结果无论是UVB辐射前还是辐射后外用黄芩苷均明显减轻紫外线辐射造成的小鼠皮肤增厚。外用黄芩苷还可减少因UVB辐射诱导的小鼠皮肤组织中过氧化氢和CPDs产量。结论黄芩苷可抵抗UVB诱导的小鼠皮肤增厚,减少光产物表达,并通过减少活性氧簇的产生而发挥抗氧化作用。

Objective To observe the effects of baicalin on UVB-mediated damages in mice skins. Methods DNA damage models of UVB irradiation- induced mice epidermis were established. The skins＇ thickness was detected. Histochemical staining was used to detect hydrogen peroxide. The immunohistochemical staining was used for CPDs detection. Results Our data demonstrated that a topical application of baicalin, either pre - or post-UVB irradiation, resulted in a significant decreasing in UVB-mediated increases in skin thickness. Further more, baicalin treatments also resulted in a significant decreasing in UVB mediated generation of H2O2 and formation of CPDs. Conclusion We suggest that baicalin could be developed as an agent for the management of conditions elicited by UV exposure including skin cancer.