2-羟基苯乙酮铜(Ⅱ)配合物与ct-DNA的键合作用研究

Study on Binding Interaction between ct-DNA and Copper(Ⅱ) Complex of 2-Hydroxy Acetophenone

以小牛胸腺DNA为靶点,通过紫外可见吸收光谱、稳态荧光发射光谱、圆二色谱和DNA粘度滴定实验,从分子水平研究了2-羟基苯乙酮铜(Ⅱ)配合物[Cu(HAP)2](1)与ct-DNA的键合方式。结果表明:配合物[Cu(HAP)2](1)主要通过插入作用和静电结合方式与ct-DNA形成键合作用,推测插入作用应基于2-羟基苯乙酮配体的芳香母环及配合物整体保持刚性平面,而对DNA聚阴离子骨架的静电结合作用则与中心铜离子的正电性有关。上述结果为深入研究2-羟基苯乙酮金属配合物的药理活性提供了科学依据。

In this study, the binding mode of a synthesized copper（ Ⅱ ） complex of 2-hydroxy acetophenone （ HAP）, [ Cu （HAP） 2 ] （ 1 ）, to calf thymus DNA （ct-DNA） as biomolecular target was discussed by UV -Vis absorption, fluorescence emission and circular dichroism spectra, as well as DNA viscosity titration experiment. Results indicated that complex 1 binds to ct-DNA mainly via intercalation and exterior electrostatic interaction, in which intercalation can be ascribed to aromatic ring of HAP and the rigid planar structure of whole complex 1, while electrostatic interaction to DNA polyanionic backbone should be attributed to the cationic copper （ Ⅱ ） center of complex 1. The research results provided scientific evidence for the further exploration of the pharmaceutical activities of serial metal complexes of HAP.