响应面法优化灵芪菌质多糖固态发酵条件

Optimization of Ganodetma lucidum-Astragalus membranaceus Polysaccharide Solid Fermentation Condition by Response Surface Analysis

采用单因素试验和响应面分析法优化灵芪菌质多糖固态发酵条件。优化后发酵条件为:采用药性固体发酵培养基,初始pH6.5,接种量10.16ml/100g,发酵温度30.65℃,相对湿度59.54%,发酵周期28d。利用此优化条件进行灵芪菌质多糖发酵,灵芪菌质多糖含量可达9.12±0.09mg/g,较优化前8.06±0.07 mg/g提高了13.15%,为开展其药效学研究及产业化研发提供理论试验依据。

Single factor test and response surface analysis were used for optimization of Ganodetma lucidum-Astragalus membranaceus polysaeeharide solid fermentation. Optimized solid fermentation conditions were as follows： herbs solid fermentation medium, initial pH6.5, inoeulum volume 10. 16ml/100g, fermentation temperature 30. 65℃, relative humidity 59.54%, fermentation period 28d. Under the above optimum conditions, Ganodetma lucidum-Astragalus membranaceus polysaeeharide （GAPS） content was up to 9. 12 ±0. 09mg/g and increased by 13.15% than unoptimization （8.06 ± 0.07 mg/g）, which provided a theoretical experimental basis for pharmaeodynamies research and industrialization development.