摘要
采用盐析、有机溶剂沉淀及DEAE- 52 柱层析的方法,提纯了绿豆胚超氧化物歧化酶(SOD) 。纯酶总活力8006 U,总蛋白量为7-3 mg,比活力为1096-8 U/mg 蛋白,纯化645-2 倍,活性回收率为44-2% 。对其理化性质分析表明:该酶的最大紫外吸收峰为260 nm ,为Cu、Zn SOD,由2 个亚基组成,亚基相对分子质量为15000。
The superoxide dismutase(SOD) from phaseolus radiatus L. embryo was purified to homogeneity by the steps including ammonium sulfate fractionation and DEAE-52 chromatography. The results showed that the enzyme was a Cu, Zn-SOD composed of two homogenous subunits of which the relative molecular mass was 15000.The specific activity of the pure enzyme was 1097 U/mg protein, the total activity was 8006 U, and the activity recovery was 42 2% . The wave length of maximum absorbing peak in ultraviolet spectrum was 260nm which was similar to that of SOD from other resources.
出处
《精细化工》
EI
CAS
CSCD
北大核心
2000年第1期57-59,共3页
Fine Chemicals
基金
内蒙古教育厅高校研究资助项目!A97044
内蒙古自然科学基金
关键词
绿豆胚
超氧化物歧化酶
性质
提纯
superoxide dismutase
phaseolus radiatus L. embryo
purification