乙型肝炎血清前S1抗原与HBV-DNA和HBeAg相关性分析

The study on the relativity among HBeAg,HBV-DNA and PreS1-antigen

目的探讨乙型肝炎血清前S1抗原(PreS1Ag)临床应用的价值。方法对365例乙型肝炎患者血清,采用酶联免疫吸附试验(ELISA)检测HBV血清标志物和PreS1Ag,用荧光定量聚合酶链反应(FQ-PCR)方法检测HBV-DNA。结果 HBV-DNA和PreS1Ag的阳性率在110例HBV大三阳中,分别为86.4%和89.1%;在66例HBV小三阳中,分别为36.4%和40.9%;在37例HBsAg、HBcAb中,分别为32.4%和41.7%;在39例HBeAg、HBcAb阳性中,分别为15.4%和15.4%;在113例HBsAb阳性中,分别为5.3%和8.0%。HBeAg、PreS1Ag阳性率随不同载量HBV-DNA升高而增高,但PreS1Ag比HBeAg增高更明显(P<0.05)。结论 PreS1Ag和HBV-DNA一样都是乙型肝炎病毒复制的敏感指标,虽然PreS1Ag和HBeAg都随HBV-DNA载量增加而升高,但PreS1Ag较HBeAg更能敏感,因此PreS1Ag具有重要的临床应用价值。

Objective To study the clinical application value of Presl-antigen. Methods The HBV markers and Presl-antigen were detected by enzyme linked immunosorbent assay （ELISA） and the HBV-DNA was detected by fluorescent quantitation poly-merase chain reaction （FQ-PCR） in 365 patients with hepatitis B. Results The positive rates of the HBV-DNA and Presl were 86.4% and 89. 1% in 110 patients of HBsAg＋, HBeAg ＋ and HBcAb ＋ ; 36.4% and 40.9% in66 patients of HBsAg ＋ ,HBeAb ＋ and HBcAb ＋ ;32.4% and 41.7% in 37 patients of HBsAg ＋ and HBcAb ＋ ; 15.4% and 15.4% in 39 patients of HeAg ＋ and HBcAb ＋ ;5.3% and 8.0% in 113 patients of HBsAb ＋. The positive rates of HBeAg and PreS1Ag increased with increasing HB^-DNA, PreS1Ag was increased significantly compared with HBeAg （ P 〈 0.05）. Conclusion Presl and HBV-DNA are the sensitive index for HBV replication, though PreS1Ag and HBeAg Presl increased with increasing HBV-DNA, PreS1Ag is more sensitive than HBeAg, and it is valuable in clinical application.