茶树接种VA菌根的生理特性研究

Study on Physiological Characteristic of Tea Plant Inoculated by VA Mycorrhiza

茶树接种VA菌根(丛枝菌根)的生理特性研究表明,茶树叶片接种VA菌根50 d后,其叶绿素含量都高于未接种的。在光合有效辐射0～1200μmol/m2.s,接种VA菌根的茶树,其净光合速率、气孔导度、蒸腾速率等均有提高;而水分利用率前期快速增加,达到高峰然后下降;茶树叶片相对含水量先上升后下降,叶片的自然饱和亏逐渐降低。接种VA菌根后,随着侵染率的增加,茶树叶片的丙二醛含量越少,脯氨酸的含量先降低后升高。VA菌根能够提高茶树保护酶的活性,在生长到90 d时,过氧化物酶(POD)活性达到最高值,超氧化物歧化酶(SOD)活性先升高再降低,但增加幅度均比较小;对过氧化氢酶(CAT)活性的影响一直高于未接种的。

The physiological characteristic of tea plant inoculated by VA mycorrhiza was studied.Results showed that 50 d after inoculation,the chlorophyll content in tea leaves inoculated by VA mycorrhiza was higher than that uninoculated by VA mycorrhiza.Results also indicated when the photosynthetical available radiation was in the range of 0～1200 μmol/m2？s,the net photosynthesis rate,the stomata conductance and the transpiration rate of the tea plant inoculated by VA mycorrhiza were all higher than those of the control,the water use efficiency（WUE） increased rapidly in earlier stage,then decreased gradually after reaching the peak,the degrees of saturation of leaves decreased gradually.After the tea plant inoculated by VA mycorrhiza,MDA content of tea leaves decreased while the hydroxyproline content decreased first and increased later with the increase of infection rate.The inoculation of VA mycorrhiza can improve the activities of protective enzymes of tea plant.During the resied of 90 d after inoculation,the activity of POD reached the peak,the activity of SOD increased slightly first and then decreased smoothly.CAT activity of tea plant treated by VA mycorrhiza was always higher than that of the control.