非小细胞肺癌组织中CD68与c-Jun蛋白的表达

Expressions of CD68 and c-Jun proteins in non-small cell lung cancer tissue

目的:探讨非小细胞肺癌(NSCLC)组织中CD68和c-Jun蛋白的表达与肺癌发生发展的关系及其意义。方法:采用免疫组化SP法检测67例人NSCLC标本(每个标本取癌、癌旁和正常组织)中CD68和c-Jun蛋白的表达,应用Spearman秩相关检验CD68阳性细胞数与c-Jun蛋白表达的相关性。结果:肺癌、癌旁及正常肺组织中CD68阳性细胞数依次为11.8±1.4、97.5±2.5和52.1±1.9,差异有统计学意义(F=52334.934,P<0.001);c-Jun蛋白的平均光密度值依次为0.25±0.03、0.23±0.02和0.20±0.01,差异有统计学意义(F=91.280,P<0.001)。癌旁组织中CD68阳性细胞数与年龄、性别、病理类型、分化程度、吸烟无关(P>0.05),与TNM分期(F=158.273,P<0.001)、淋巴结转移(F=84.985,P<0.001)、是否侵及胸膜(F=16.422,P<0.001)有关。癌旁组织中c-Jun蛋白的表达与年龄、性别、病理类型、吸烟无关(P>0.05),与TNM分期(F=19.319,P<0.001)、分化程度(F=33.918,P<0.001)、淋巴结转移(t=12.155,P<0.001)、是否侵及胸膜(F=8.318,P=0.005)有关。在癌旁组织中CD68阳性细胞数与c-Jun蛋白的表达呈正相关(rs=0.519,P<0.001)。结论:巨噬细胞主要浸润在癌旁组织中,可能通过激活蛋白-1促进NSCLC的发展转移。

Aim：To explore the expressions of CD68 and c-Jun proteins in non-small cell lung cancer（NSCLC）,and their relationship with the development of lung cancer.Methods：Immunohistochemical SP method was used to detect the expression of CD68 and c-Jun proteins in 67 cases of NSCLC samples including cancer tissues,adjacent tissues and normal tissues,and Spearman rank correlation analysis was used to investigate the correlation of CD68＋cell number with the expression of c-Jun protein.Results：CD68＋cell number in cancer tissues,adjacent tissues and normal tissues was 11.8±1.4,97.5±2.5,52.1±1.9,respectively,and there was statistical significance（F=52 334.934,P0.001）.The mean optical density of c-Jun protein in cancer tissues,adjacent tissues and normal tissues was 0.25±0.03,0.23± 0.02,0.20±0.01,and there was statistical significance（F=91.280,P0.001）.The CD68＋cell number in adjacent tissues was correlated with the TNM stage（F=158.273,P0.001）,lymph node metastasis（F=84.985,P 0.001）and involvement of pleura（F=16.422,P0.001）.The expression of c-Jun in adjacent tissues was correlated with TNM stage（F=19.319,P0.001）,the differentiation of lung cancer（F=33.918,P0.001）,lymph node metastasis（F=12.155,P0.001）and involvement of pleura（F=8.318,P=0.005）.In adjacent tissues CD68＋cell number was positiuely correlated with the expression of c-Jun protein（r s =0.519,P0.001）.Conclusion：Macrophages mainly infiltrated in tumor-adjacent tissues,and they may promote the development and metastasis of NSCLC by activating AP-1.