多发性硬化患者血清激肽释放酶1和6水平分析

Determination of serum kallikrein 1 and kallikrein 6 levels in patients with multiple sclerosis

目的:测定多发性硬化(MS)初发组与复发组患者血清激肽释放酶1(klk1)与激肽释放酶6(klk6)的水平,研究二者与MS复发的关系。方法:采用ELISA法测定健康对照者、其他神经系统疾病患者、初发MS患者和复发MS患者各50名外周血血清klk1和klk6水平。结果:4组患者血清klk1和klk6水平比较,差异均有统计学意义(F=158.940和61.490,P<0.001);MS初发组与复发组血清klk1和klk6水平均高于其他2组,且MS复发组高于初发组(P<0.05)。MS患者血清klk1、klk6水平分别与MS的复发呈正关联(rP=0.555和0.447,P<0.05)。结论:血清klk1、klk6水平与MS复发相关,可能作为反映MS复发的指标。

Aim：To determine the serum levels of kallikrein 1（klk1）and kallikrein 6（klk6）in the multiple sclerosis （MS）patients with initial attack and patients with recurrent attack,so as to investigate the role of klk1 and klk6 in the relapse process of MS.Methods：ELISA method was used to detect the klk1 and klk6 levels in the serum of 50 MS patients with initial attack and 50 MS patients with recurrent attack,as well as other neurological diseases controls and normal controls.Results：The serum klk1 and klk6 levels among the 4 groups had significant differences（F=158.940 and 61.490,P0.001）.The klk1 and klk6 levels in the serum of the MS patients with initial attack and MS patients with recurrent attack were significantly higher than those of the other 2 groups（P0.05）,and those of recurrent group were also significantly higher than the initial group（P0.05）.Serum klk1 and klk6 levels and disease recurrence were positively correlated （r P =0.555 and 0.447,P0.05）.Conclusion：The serum levels of klk1 and klk6 have correlation with the relapse of MS,and klk1 and klk6 can be served as markers to reflect the relapse of MS.