玻璃化冷冻法对冻融胚胎移植周期结局的影响

Effects of vitrification methods on clinical outcome of frozen-thawed embryo transfer

目的:探讨玻璃化冷冻法对冻融胚胎移植周期结局的影响。方法:对324个玻璃化冷冻人卵裂期胚胎复苏周期和146个程序化慢速冷冻胚胎复苏周期进行分析,比较2组冷冻胚胎复苏后的存活率、完整率、种植率、临床妊娠率、孕周、新生儿身高及体质量等指标。结果:玻璃化冷冻组的胚胎完整率、临床妊娠率均高于程序化慢速冷冻组(83.24%vs47.54%,χ2=179.772,P<0.001;37.38%vs27.66%,χ2=4.073,P=0.044);2组流产率、分娩孕周、新生儿出生体质量及身高比较,差异无统计学意义(P均>0.05)。结论:玻璃化冷冻胚胎较程序化慢速冷冻法有更高的复苏胚胎完整率及临床妊娠率,可在临床上安全应用。

Aim：To investigate the effects of vitrification methods on the clinical outcome of frozen-thawed embryo transfer.Methods：A statistical analysis was performed on 324 vitrification cycles and 146 slow-freezing cycles.After warming,the survival rate,blastomere integrity rate,implantation rate,clinical pregnancy rate,gestational weeks,newborn weight and height were evaluated and compared between the two methods.Results：The blastomere integrity rate and clinical pregnancy rate by vitrification were significantly higher than those by slow-freezing method（83.24%vs 47.54%,χ2= 179.772,P0.001;37.38%vs 27.66%,χ2=4.073,P=0.044）.There were no significant differences between the two methods in the miscarriage rate,gestational weeks,newborn weight and height（P0.05）,respectively.Conclusion：Vitrification provides a higher blastomere integrity and clinical pregnancy rate than slow-freezing method.It could be safe in clinical application.