摘要
目的:从卵白蛋白酶解液中分离抗氧化肽。方法:通过超滤、离子色谱和反相高效液相色谱法从卵白蛋白酶解液中分离制备抗氧化肽,用电喷雾质谱肽的结构进行表征。结果:分离纯化获得抗氧化能力较强的3个肽:OPQ3-1、OPQ3-2和OPQ3-4,分别是源于鸡蛋卵白蛋白第115～117氨基酸残基(Pro-Glu-Tyr),分子质量408.14Da;第245～248氨基酸残基(Leu-Pro-Asp-Glu),分子质量473.19Da;第148～150氨基酸残基(Trp-Val-Glu),分子质量433.18Da。结论:卵白蛋白经胃蛋白酶水解获得抗氧化多肽。
Objective：Isolation antioxidative peptide from ovalbumin by enzymatic hydrolysis. Method：Antioxidative peptides in the hydrolysates were purified by ultrafiltration（UF） membrane system, Q-Sepharose FF ion-exchang chromatography and reverse phase high-performance liquid chromatography（RP-HPLC）. Their structure was further identified by electrospray ionization tandem mass spectrometry （ESI-MS/MS）. Result：Three peptides with strong antioxidative activities were purified from the hydrolysates. Their structure were Pro-Glu-Tyr （MW：408.14Da）, Leu-Pro-Asp-Glu（MW： 473.19 Da） and Trp-Val-Glu（MW： 433.18）, which are 115～117th, 245～248th and 148～150th amino acid residuces in the sequence of ovalbumin, respectively. Conclusion：Three antioxidative peptides were obtained from hydrolysates of ovalbumin by pepsin.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2011年第8期16-22,共7页
Journal of Chinese Institute Of Food Science and Technology
基金
江西省教育厅项目(GJJ98134)
关键词
卵白蛋白
酶解
抗氧化肽
分离
ovalbumin
enzymatic hydrolysis
antioxidative peptide
isolation
