高效液相色谱-示差折光检测法测定补中益气丸中蜂蜜的含量

HPLC-RD determination of honey in Buzhong Yiqi Wan

目的建立补中益气丸(蜜丸)蜂蜜中葡萄糖、果糖、蔗糖、麦芽糖含量测定方法,以监控补中益气丸质量。方法 采用高效液相色谱-示差折光检测(HPLC-RD)法;色谱柱为Prevail Carbohydrate ES(250mm×4.6mm,5μm);流动相为乙腈-水(75∶25);流速1.0mL·min-1;柱温:35℃。结果葡萄糖、果糖、蔗糖、麦芽糖对照品分别在17.868～179.14、16.144～161.44、3.016～30.16、6.565～98.475μg线性关系良好,4种成分加样回收率分别为97.1%、101.9%、102.6%、95.9%,RSD分别为0.8%、2.0%、0.8%、1.3%(n=6)。结论该方法操作简便,灵敏度高,重复性好,可用于补中益气丸中蜂蜜的含量测定。

Objective To evaluate the quality of Buzhong Yiqi Wan （honey pills） to determine the contents of glucose, fructose, sucrose and maltose. Methods An HPLORD system was adopted with a Prevail Carbohydrate ES （250 mm ×4.6 mm, 5 μm）. The mobile phase was acetonitrite-water （75 ： 25）, the flow was 1.0 mL · min^-1 , and the column temperature was 35 ℃. Results The standard curves of glucose, fructose, sucrose and maltose showed good linearity over 17. 868-179.14, 16. 144-161.44, 3. 016-30.16, and 6. 565-98. 475μg, respectively. The average recovery was 97. 1%, 101.9%,102.6%,and 95.9%, with RSD=0. 8%, 2.0%, 0.8%and 1.3%（n=6）, respectively. Conclusion The method is simple, sensitive, reproducible, and effective for the determination of honey in Buzhong Yiqi Wan （honey pills）.