黄荆子提取物对人卵巢癌CoC1细胞生长的影响

Effect of the Extract of the Vitex Negundo Seed on the Growth of Human Ovarian Cancer CoC1 Cell Line

目的观察4种黄荆子提取物(EVn-10、EVn-20、EVn-30、EVn-50)对人卵巢癌CoC1细胞生长的影响,筛选出抑制增殖活性最强的提取物。方法体外培养人卵巢癌CoC1细胞,分为4个实验组及3个对照组,以不同浓度的提取物及对照药物孵育48 h,MTT比色法测定四种提取物对人卵巢癌CoC1细胞增殖活性的影响。结果 4种黄荆子提取物对人卵巢癌CoC1细胞增殖活性均有抑制作用,以EVn-50效价最高,其IC50为1.02μg/mL,效价强度与DDP(IC50为0.82μg/mL)接近。结论黄荆子乙酸乙酯提取物EVn-50能有效抑制人卵巢癌CoC1细胞增殖和生长。

Objective To investigate the therapeutic effect of four species of extracts from Vitex negundo seed（ EVn- 10,EVn-20,EVn-30,EVn-50） on human ovarian cancer CoCl cell line in vitro,thus to get the most effective one. Methods CoCl cells that cultured in vitro were divided into four experimental groups （EG） and three control groups （CG） ; Cells were treated with different concentrations extract in EG and CG done with control drug ;The viability of CoCl cells was determinated by MTT assay. Results Four species of extracts of Vitex negundo seed all inhibited viability of CoCl cells. The acetoacetate extract of Vitex negundo seed（EVn-50） was the strongest in those,and its IC50 value was 1.02μg/mL. It was near to DDP（ IC50 value was 0. 82μg/mL）. Conclusions The acetoacetate extract of Vitex negundo seed（EVn-50） can inhibit viability and growth of human ovarian cancer CoCl cell line.