摘要
对液相色谱法测定含乳饮料中安赛蜜进行了方法优化,对色谱条件进行了调整,采用ODS—Cl8型(150mmx4.6 mm)色谱柱,流动相甲醇—0.02mol/L乙酸铵(体积比5:95),流速为1.0 mL/min,采用紫外检测器,检测波长为214nm。该方法测定结果的相对标准偏差为1.04%(n=4),平均回收率为91.33%,该法满足实验要求。
The method of determination of Acesulfame K in milk beverage by high performance liquid chromatography was optimized.The HPLC condition was adjusted accordingly.The separation was achieved by using a ODS-C18 column（150 mm ×4.6 mm）with mobile phase 0.02 mol/L NH4Ac/methanol（95/5） and velocity of 1.0 mL/min.Lastly the determination was conducted at 214 nm through a UV detector.The relative standard deviation of measuring results of the method was 1.04%（n = 4）,the average recovery was 91.33%.The method could meet the requirements.
出处
《食品与发酵科技》
CAS
2011年第6期79-80,99,共3页
Food and Fermentation Science & Technology
