FHA/Mg-Zn合金体内降解及生物相容性的评价

In vivo degradation and biocompatibility of fluoridated hydroxyapatite coated Mg-Zn alloy

目的观察氟磷灰石﹙FHA﹚涂层镁锌合金在动物体内降解和生物相容性。方法取8只新西兰健康成年大白兔,随机分为2组:氟磷灰石﹙FHA﹚涂层镁锌合金和无涂层镁锌合金。在动物一侧股骨髁使用克氏针钻洞﹙4.5mm×10mm﹚,将FHA/Mg-Zn合金的棒状物﹙4.5mm×10mm﹚植入,同样办法在另一只兔子股骨髁植入大小一样的Mg-Zn合金的棒状物,术后4周取动物心、肝、肾及关节囊,HE染色后观察组织病理学改变,取股骨髁组织切片品红苦味酸染色,观察植入物的降解及新骨的形成。股骨髁行Micro-CT分析。结果 2组材料对动物心、肝、肾及关节囊的组织病理结果均正常。FHA/Mg-Zn合金与周围骨组织紧密接触,周围有大量新骨形成,未见明显降解。Mg-Zn合金明显降解,与周围骨组织形成缝隙,新骨形成较少。结论 FHA/Mg-Zn合金有效控制降解,具有良好生物相容性。

Objective To investigate the degradation and biocompatibility of fluoridated hydroxyapatite coated Mg-Zn alloy in vivo.Method A total of 8 New zealand rabbits were randomly divided into fluoridated hydroxyapatite ﹙FHA﹚ coated Mg-Zn alloy group and no-coated Mg-Zn alloy group.The condyles of femur were drilled ﹙φ4.5mm×10mm﹚ and implanted with same magnesium alloy.At 4 weeks post-operatively,tissues of heart、liver、kidney and joint capsule were observed by HE staining,and degradation of material were observed.Bone formation were assessed by fuchsin trinitrophenol staining.At the same time,condyles of femur were analysed using Micro-CT.Results Pathology of heart、liver、kidney and joint capsule were normal and there were no significant abnormalities in 2 groups.Compared with Mg-Zn alloy group,new bones in FHA/Mg-Zn alloy group were more and had tighter binding with the magnesium alloys.In addition,FHA/Mg-Zn alloy had no significant degradation.Conclusion FHA-coated magnesium alloy can effectively control degradation and have good biocompatibility.