芥菜及其原始亲本种的酸性磷酸酶同工酶分析

ANALYSIS OF ACID PHOSPHATASE ISOZYMES IN BRASSICA JUNCEA COSS(L.) AND THEIR ORIGINAL PARENTAL SPECIES

本研究利用聚丙烯酰胺凝胶电泳法研究了芥菜(Brassica juncea Coss)不同类型品种和其原始亲本种白菜(Brassica campestris L.)、黑芥(Brassi-ca nigra Kock)的酸性磷酸酶同工酶酶谱的表现,分析了芥菜的起源与白菜、黑芥的关系。研究结果表明,芥菜的酸性磷酸酶同工酶酶谱表现与白菜、黑芥的酸性磷酸酶同工酶酶谱具有较强的同源性。作者初步认为菜用芥菜的原始亲本种很可能是起源于中国的野生型黑芥(伊犁油菜)和白菜。 芥菜不同类型品种的酸性磷酸酶同工酶酶谱表现出一定的差异,特别是APS—Ⅰ和APS—Ⅱ两个酶区,品种间差异明显。APS—Ⅰ比APS─Ⅱ表现更加稳定,APS—Ⅰ可做为标记酶区在芥菜种质资源研究和育种实践中加以利用。不同类型白菜的酶谱表现差异较小。供试大白菜(B.campestris L.ssp.pekinesis(Lour)Olsson)品种间酶谱表现完全一致,菜心(B.cam-pestris ssp.chinensis var.parachinensis)的酶谱表现与大白菜的谱带基本一致,仅小白菜(B.campestris L.ssp.chinensis(L.)M.)的酶谱表现与大白菜有一定的差异。

This analysis of acid phosphatase was carried out in different Cul-tivars of BraSsica juncea and their original parental species, Brassica campestris, B. nigra. The origin of vegetable mustard was discussed. The acid phosphatase isozymes zymogram of B. juncea showed very closely related to B. campestris and B. nigra. It is very possible that the original parental species of vegetable mustard are wild black mustard and primitive Chinese cabbage derived from China. The isozyme zymogram showed conspicuous differences among mustard cultivars, especially for Aps-Ⅰ region and Aps-Ⅱ region. The results suggested that Aps-Ⅰ region could be used as genetic marker region in mustard germplasm research and utilization. The isozyme zymogram showed few differences among cultivars in B. campestris.