同时蒸馏萃取法与水蒸气蒸馏法分离分析烟草挥发性、半挥发性中性成分的比较

Comparison of SDE and WSD in Analyzing the Volatile,Semi-Volatile Neutral Components in Tobacco

使用同时蒸馏萃取法与水蒸气蒸馏法对烟草中挥发性、半挥发性中性成分进行了气相色谱分析和气相色谱 /质谱鉴定。结果显示 ,两种方法分析烟草中挥发性、半挥发性中性成分的重复性相差不大。同时蒸馏萃取法测得的回收率总体上比水蒸气蒸馏法高。水蒸气蒸馏法较适合分析沸点较低的挥发性、半挥发性中性成分 ,而同时蒸馏萃取法比水蒸气蒸馏法分析烟叶样品中挥发性、半挥发性中性成分的应用范围广 ,特别是分析烟叶样品中沸点较高的挥发性、半挥发性中性成分更为有效。同时蒸馏萃取法比水蒸气蒸馏法步骤简单、省时、省工、节约溶剂。

The volatile,semi\|volatile neutral components in tobacco were respectively extracted by using the simultaneous distillation and extraction method (SDE) and water steam distillation method(WSD),and determined with GC and GC/MS. The quantitative results of these neutral components were compared.The difference of the repeatability of the two methods is little.Generally, the recovery of SDE is higher than that of WSD.WSD is suitable to determine the volatile,semi\|volatile neutral components whose boiling points are lower. The neutral components that have wide boiling point range can be analyzed with SDE. Specifically,SDE is more suitable to determine the high boiling point neutral components than WSD.There are several advantages in the analysis of the neutral components in tobacco with SDE,including simplicity,fast,saving labor and solvent.In addition,the determination of neutral components with SDE was carried out with different solvent and time to get the best analyzing conditions.