摘要
目的:探讨芍药苷对6羟基多巴胺(6-OHDA)致大鼠肾上腺嗜铬细胞瘤细胞(PC12)的细胞损伤保护作用及其可能机制。方法:体外培养PC12细胞,用6-OHDA建立细胞损伤模型。MTT和乳酸脱氢酶(LDH)活性测定存活率;RT-PCR检测Bcl-2、Bax的mRNA表达,Hochest33342/PI双染检测细胞凋亡率。结果:25、50、100μmol.L-1芍药苷可显著减少6-OHDA诱导的PC12细胞凋亡,降低LDH漏出率,增加Bcl-2 mRNA和减少Bax mRNA表达。结论:芍药苷可显著减少6-OHDA诱导的PC12细胞凋亡,其作用机制可能与调节Bax、Bcl-2表达有关。
Objective： To investigate the protective effects of paeoniflorin（PF） on PC12 cells injury by 6-hydroxydopamine（6-OHDA） and its possible mechanism.Methods： PC12 cells were treated with 6-OHDA to set up the damaged cell model.Cell survival was detected by MTT and lactate dehydrogenase.The mRNA expression of Bcl-2 and Bax were detected by RT-PCR.The apoptosis was monitored with Hoechst 33342/PI staining.Results： Paeoniflorin can significantly decrease 6-OHDA-induced LDH leakage rate and apoptosis.Paeoniflorin could increase the expression of Bcl-2 mRNA,and decrease the expression of Bax mRNA.Conclusion： Paeoniflorin inhibited 6-OHDA-induced apoptosis in PC12 cells.The mechanism is related to the regulation of mRNA expression of Bcl-2 and Bax in PC12 cells.
出处
《药学与临床研究》
2011年第6期511-514,共4页
Pharmaceutical and Clinical Research
