摘要
采用高效液相色谱法,色谱柱:Turner C18柱;流动相:乙腈-水(65∶35);流速:1.0 mL.min-1;柱温:30℃;检测波长:322 nm;进样量:10μL。结果显示,蛇床子素在0.49～49μg.mL-1的浓度范围内与峰面积呈良好的线性关系(r=0.9999),平均回收率(n=6)为96.33%,RSD为2.80%。
An HPLC method was adopted.The determination was performed on a Turner C18 column with the mobile phase consisted of acetonitrile-water（65∶35） at a flow rate of 1.0 mL·min-1.The detection wavelength was set at 322 nm and the column temperature was 30℃.The linear range of osthol was 0.49~49 μg·mL-1（r=0.9999） with an average recovery of 96.33%（RSD=2.80%,n=6）.
出处
《药学与临床研究》
2011年第6期567-568,共2页
Pharmaceutical and Clinical Research
基金
苏州市科技局科技立项研究课题(SYSD2010092)
