DNA聚合酶Ⅰ的功能与结构的发现

Discovery of Function and Structure of Deoxyribonucleic Acid PolymeraseⅠ

本文具体介绍了DNA聚合酶Ⅰ的功能与结构的发现过程,包括聚合酶活性中心模型的提出;聚合酶分子量的测定;聚合酶的分离及其活性片段的获得;聚合酶Klenow片段晶体结构的测定以及DNA结合部位与活性位点的确立;Klenow片段结合DNA的晶体结构以及噬菌体T7DNA复制的晶体结构的测定,在测定这些复合物共晶体的基础上,提出了DNA与聚合酶结合的右手模型。

The process of finding the function and the structure of DNA polymerase is introduced.The key events include the proposition of active center model of the polymerase,the determination of molecular weight of the polymerase,the dissociation of the polymerase and achievement of active fragment,the determination of crystal structure of Klenow fragment binding DNA and of bacteriophage T7DNA replication.Based on the determined crystal structure,a right hand model was proposed.