HPLC法同时测定五味子饮片中6个木脂素类成分的含量

HPLC simultaneous determination of six lignans in Fructus Schisandrae

目的:建立HPLC法同时测定五味子中五味子醇甲、五味子醇乙、五味子酯甲、五味子甲素、五味子乙素和五味子丙素6个木脂素类成分含量。方法:采用依利特C18(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈(A)-水(B),梯度洗脱(0～17min,50%A;17～25 min,50%A→55%A;25～30 min,55%A→75%A;30～35 min,75%A;35～40 min,75%A→65%A;40～45min,65%A→50%A),流速1.0 mL.min-1,检测波长217 nm,柱温30℃。结果:6个被测木脂素类成分分离度良好;各成分质量浓度与峰面积在测定范围内均呈良好的线性关系(r>0.9995);平均加样回收率(n=9)分别为99.8%,100.7%,101.6%,100.3%,100.5%,100.4%。结论:本研究所建立的HPLC方法稳定可靠,简便可行,可用于五味子中木脂素类成分的同时定量分析,为五味子药材及其炮制品的质量评价和质量控制奠定了基础。

Objective：To establish an HPLC method for simultaneous determination of schisandrin,schisandrol B,schisantherrin A,deoxyschisandrin,schisandrin B and schisandrin C in Schisandra chinensis.Method：Elite C18 column（250 mm×4.6 mm,5 μm） was adopted.Acetonitrile（A）-water（B） was used as gradient mobile phase（0-17 min,50%A;17-25 min,50%A→55%A;25-30 min,55%A→75%A;30-35 min,75%A;35-40 min,75%A→65%A;40-45 min,65%A→50%A） at the flow rate of 1.0 mL·min-1.The detection wavelength was 217 nm and the column temperature was 30 ℃.Results：Excellent chromatographic separation was achieved for six studied lignans with good linearity（r〉0.9995） over the studied concentration ranges.The average extraction recoveries（n=9） were 99.8% for schisandrin,100.7% for schisandrol B,101.6% for schisantherrin A,100.3% for deoxyschisandrin,100.5% for schisandrin B and 100.4% for schisandrin C.Conclusion：The HPLC method developed for simultaneous determination of six lignans is simple and valid.And It can be used for quality evaluation and quality control of Schisandra chinensis and its processing products.