高效液相色谱-质谱检测红葡萄皮中花色苷

HPLC-DAD-MS-MS determination of anthocyanins in red grape skin

目的:建立高效液相色谱-质谱联用(LC-MS)方法,检测红葡萄皮中花色苷。方法:采用AichromBond C18色谱柱(250mm×4.6 mm,5μm),流动相A(2%甲酸水溶液)和B(乙腈)梯度洗脱(0 min:10%B;10 min:25%B;15 min:30%B;35 min:50%B)。流速:0.6 mL.min-1,检测波长:530 nm,二极管阵列检测器,质谱检测设定为负离子模式。结果:大泽山玫瑰香红葡萄皮中主要花色苷有15种。结论:LC-MS法检测红葡萄皮中花色苷,具有高的灵敏度和选择性,方法准确可靠。

Objective：To establish an HPLC-DAD-MS-MS method for determination of anthocyanins from red grape skin.Methods：The major anthocyanins of grape skin was determined by HPLC-DAD-MS-MS method.AichromBond C18 column（250 mm×4.6 mm,5 μm） was used in HPLC assay.Mobile phase A and B were 2% formic acid and acetonitrile,respectively.0 min：10% B;10 min：25% B;15 min：30% B;35 min：50% B.The flow rate was 0.6 mL·min-1.The detective wavelength was 530 nm.The PDA detector and negative ion mode of MS were adopted.Results：15 anthocyanins were determined from the red grape skin of Mountain Daze.Conclusion：It is sensitive and selective to determine anthocyanins of grape skin with HPLC-MS method.The method is also accurate and reliable.