蛋鸡日粮含苏丹红Ⅳ的鸡蛋内源性代谢物差异性研究

Study on Significantly Differences of Endogenous Metabolites in Eggs which Obtained from Layers Fed with Sudan Ⅳ

采用高分辨Agilent 6530液相色谱-四极杆/飞行时间质谱联用技术,对喂食日粮中含苏丹红Ⅳ(0、100、150、200mg/kg)的海兰褐蛋鸡的鸡蛋进行代谢组学研究,即对喂养28d后的鸡蛋样品分别在电喷雾离子源(electrospray ionization,ESI)正、负离子模式下进行检测,并通过相关代谢组学统计分析软件(mass profiler professional,MPP)对采集到的谱图进行主成分分析,从而获得蛋鸡食用苏丹红Ⅳ后鸡蛋中的代谢物信息。结果表明,该液质联用技术可将空白对照组与喂食苏丹红Ⅳ组进行明显的区分,并可借助数据库中23000种代谢物信息对发生显著变化(P<0.05,倍数变化≥2)的差异性代谢物组分进行初步的鉴定。研究结果为深入进行苏丹红Ⅳ的代谢组学研究提供了依据。

Metabolomics/metabonomics s tudy of Sudan Ⅳ was depend on the eggs of Highland Brown layers with determination by Agilent 6530 liquid chromatography-quadrupole/time of flight mass spectrometer（LC-MS/Q-TOF）,which was high resolution mass spectrmeter with electrospray ionization（＋/-）.The egg samples were obtained from hens which were fed Sudan Ⅳ（0,100,150,200 mg/kg）and were taken after 28 days-feeding.Then endogenous metabolites＇information of eggs could be acquired by principal component analysis of statistical analysis software（mass profiler professional,MPP）.Results showed that,this method could distinguish blank control group and the feeding of Sudan Ⅳ group clearly.Endogenous metabolites which have significantly differences（P0.05,fold change≥2） had been preliminary identified by searching in database with 23000 metabolites.The results provided a basic work for further studies of metabolomics/metabonomics of Sudan Ⅳ.