三叶草叶蛋白提取工艺优化研究

Experimental Study on Parameter Optimization of Extraction Process of Leaf Protein from Clover

作者选用三叶草为试验材料,通过正交试验L9(34)分别对料水比、加盐比、不同pH和絮凝温度4个因素进行优化。以叶蛋白提取率、蛋白质量分数为指标,以期获得三叶草叶蛋白提取的最佳优化工艺参数。正交试验表明,提取三叶草叶蛋白的最佳提取工艺为A3B3C1D2,即料水比为1∶3、加盐量为5%、pH为3.0、絮凝温度为70℃为最佳的提取工艺组合。本研究初步用正交试验的方法对三叶草叶蛋白的最佳提取工艺进行了优化研究,为三叶草叶蛋白的开发应用提供了理论依据。

In this paper,the author select several common plants as the experimental materials,which are Clover.Then through Orthogonal Test L9（34）,we partly optimize the parameters using the leaf protein concentrates（LPC） extraction technology,which are ratio of material to water,salt concentration,pH of extracting solution and coagulating temperature.we will obtain the optimized technological parameters in the leaf protein concentrates extraction technology.The results indicated that while in the orthogonal experiment,the ratio of clove meal to water,pH for extraction solution and coagulation temperature were affected LPC extraction ratio respectively.The optimum combination factor for clove extracting LPC were that 1∶3 for ratio of clove meal to water,5% for salt concentration,pH 3.0 for extraction solution and 70 ℃ for coagulation temperature,this research studied the optimum extraction technology of LPC by orthogonal test,so as to provide the theory for the development and application of LPC.