致犊牛肺炎和关节炎牛支原体新疆株的分离与鉴定

Isolation and Identification of Mycoplasma bovis Xinjiang Strain Inducing Pneumonia and Arthritis of Calf

自2010年7月以来,新疆北疆部分地区某些奶牛场犊牛陆续出现严重的肺炎和关节炎,为确定其病原,无菌采集8个牛场病死犊牛的肺脏和关节液,患病犊牛的血清和部分鼻拭子,病料接种筛选培养基,通过菌落形态观察、特异性PCR鉴定、生长抑制试验和血清学检测,确定牛支原体15株,其中7株牛支原体克隆测序,与PG45 OPP F基因同源性为97.32%～100.00%。牛支原体ELISA试剂盒检测92份血清显示,阳性率82.61%。结果表明,该地区奶牛场发病犊牛以牛支原体感染为主。

Since July 2010,a serious pneumonia and arthritis occurred in the calves of some dairies in north area of Xinjiang.In order to detect the pathogen,the died calf lungs and joint fluid,the sick calf serum and some nasal swabs was collected with asepsis from 8 dairies.Add the samples was inoculated the selected material medium.The 15 Mycoplasma bovis（M.bovis） isolates were identified by colony morphology,specific PCR,growth inhibition test（GIT） and serological test,7 isolates were cloned and sequenced,the homology of nucleotide sequences were 97.32% to 100.00% in OPP F gene with PG45 strain.Furthermore,92 serum samples were detected by ELISA,and the positive rate was 82.61%.The results indicated that the disease was primarily caused by M.bovis.