五华鸡J亚群白血病病毒env部分基因的克隆和序列分析

DNA Cloning and Sequence Analysis of Partial env Gene of Avian Leukosis Virus Subgroup J in Wuhua Chicken Flocks

本试验通过PCR技术获得了安徽省地方品种五华鸡禽白血病病毒J亚群(avian leukosis virus subgroup J,ALV-J)env部分基因序列ALV-J-env1,并将该序列与GenBank数据库中登录的8条env基因相应序列进行了比对分析。结果表明,五华鸡已经感染了J亚群禽白血病,且部分鸡个体已经发病。通过分析可见,ALV-J-env1与所比较的基因序列同源性介于94.2%～96.6%之间,说明不同毒株之间的env基因有一定变异。但与ALV-J-env1同源性最高的是来自于中国ALV-J毒株的HQ425636和HM235665基因序列,且在进化树中聚集为一组,暗示它们之间亲缘关系较近,由共同的毒株进化而来。与ALV-J-env1同源性最低的是来源于马来西亚毒株的AY312965基因序列,遗传进化分析也进一步证实,两者之间亲缘关系较远。

The partial sequence of avian leukosis virus subgroup J（ALV-J） env gene was cloned by PCR in Anhui province,which was named as ALV-J-env1.And we compared ALV-J-env1 with the partial sequence of eight ALV-J env genes in the GenBank database.The results showed that ALV-J infection in Wuhua chicken was found in Anhui province.Alignment of nine gene sequences of ALV-J env,the homology between ALV-J-env1 and the other sequences ranged from 94.2% to 96.6%.The result revealed that the env gene from different strains had the mutation at nucleotide sequence.However,the highest homology with ALV-J-env1 were HQ425636 and HM235665 gene sequences isolated from strains in China,and they gathered together in the evolutionary tree,indicating a close genetic relationship,and they evolved from common strains.The lowest homology with ALV-J-env1 was the gene sequence of AY312965 isolated from Malaysia,it could be further confirmed by phylogenetic analysis.