茵陈色原酮对小鼠急性乙醇性肝损伤的保护作用研究

Hepatoprotection and Mechanism of Capillarisin in Acute Alcoholic Liver Injury in Mice

目的:探讨茵陈色原酮对小鼠急性乙醇性肝损伤的保护作用及其机制。方法:实验小鼠随机分为5组:模型组、茵陈色原酮(高、中、低剂量)组和正常对照组,每组各6只。给予茵陈色原酮组小鼠相应剂量的茵陈色原酮灌胃7d,第7天时将模型组、茵陈色原酮组小鼠采用50%乙醇12mL/(kg·d)灌胃,禁食12h,致小鼠急性乙醇性肝损伤;观察小鼠肝脏组织形态,计算肝脏指数(LI),ELISA法测定小鼠血清丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)、总蛋白(TP)、肝胞浆乙醛脱氢酶(ALDH)、谷胱甘肽过氧化物酶(GSH-Px)水平。结果:茵陈色原酮(高、中剂量)组均明显降低急性乙醇性肝损伤小鼠肝脏指数(P<0.01);高剂量组可减轻肝组织脂肪变性(P<0.05),明显降低血清ALT、AST水平(P<0.01),升高肝胞浆ALDH、GSH-Px水平(P<0.01)与血清TP水平(P<0.01)。结论:茵陈色原酮对小鼠急性乙醇性肝损伤具有保护作用,其机制与增强肝脏清除乙醛和抗氧化能力有关。

Objective： To investigate the hepatoprotection and mechanism of capillarisin against acute alcoholic liver injury in mice. Methods： To give capillarisin mixed with distilled water orally to mice in the capillarisin group for 7 days; in the 7th day to give 12 mL/（kg, d） of 50% ethanol to the model group and the capillarisin group, fasting 12 h, resulting in acute alcoholic liver injury. After that the morphology of liver tissue was observed, the liver index was calculated, the levels of serum alanine aminotransferase（ALT）, aspartate aminotransferase（AST）, total protein （TP）, liver cytoplasmic aldehyde de-hydrogenase（ALDH） and glutathione peroxidase（GSH-Px） were determined by ELISA. Results： The liver index of acute alco-hol-induced liver injury in mice could significantly be reduced in capillarisin large and medium-dose group（P〈0.01）, liver ste-atosis could be reduced in large-dose group （P〈0.05）, serum ALT, AST levels could be significantly reduced（P〈0.01）, liver cytosolic ALDH, GSH-Px levels could be elevated（P〈0.01）, and serum TP levels could be elevated（P〈0.01）. Conclusion： Capillarisin had protective effect on acute alcoholic liver injury in mice and its mechanism had correlation with accelerating the liver＇s ability to remove acetaldehyde and enhancing liver antioxidant capacity.