大蒜芦荟液消除R质粒的实验研究

Experimental study of garlic mixture with aloe vera on R-plasmid elimination

目的:探讨大蒜芦荟混合液对大肠杆菌耐氯霉素(CM)R质粒和痢疾杆菌耐氯霉素(CM)R质粒消除作用。方法:将大蒜芦荟混合液作为R质粒消除剂,消除痢疾杆菌耐CM质粒和大肠杆菌耐CM质粒。结果:经大蒜芦荟液作用的大肠杆菌和痢疾杆菌在含CM中国蓝培养基和含CMSS培养基均不生长,但在不含CM中国蓝培养基和不含CMSS培养基都能生长。结论:大蒜芦荟混合液对大肠杆菌和痢疾杆菌的R质粒在体外均具有消除作用,说明大蒜芦荟液有预防肠道菌耐药性作用。

Objective：To investigate the effects of garlic mixture with aloe vera on resistant plasmid elimination from Escherichia coli and Shigella flexneri chloramphenicol（CM） resistance（R）.Methods：The garlic mixture with aloe vera was used as R-plasmid elimination reagent to eliminate CM R-plasmids of Shigella flexneri and Escherichia coli.Results：The CM resistant Escherichia coli and Shigella flexneri treated by the garlic aloe liquid did not grow in China blue agar medium and CMSS culture medium containing CM,but grew in those media absent of CM and CMSS.Conclusion：The garlic mixture with aloe vera eliminated the R-plasmids from Escherichia coli and Shigella flexneri in vitro suggests that this reagent can prevent intestinal bacteria from antibiotic resistance.