异齿裂腹鱼人工规模化繁殖技术研究

Study on the large scale artificial propagation of Schizothorax o′connori

2010年4～6月,对野生异齿裂腹鱼(Schizothorax o'connori)人工规模化繁殖技术进行研究,并初步进行产后亲鱼恢复培养技术研究。对108尾雌鱼进行干法人工授精,共采卵104万多粒,孵出仔鱼62万多尾。其中45尾雌亲鱼自然成熟,共采卵46.8万多粒,平均受精率和孵化率低于人工催产雌鱼卵。人工催产83尾雌亲鱼,催产率53.3%～86.4%,采卵57万多粒。人工催产的亲鱼部分能自然产卵、受精。催产剂组合为:混合使用地欧酮(DOM)和促黄体素释放激素类似物(LHRH-A3),采取1针注射,每kg雌鱼体重注射剂量为(10～12)mg DOM+(20～24)μg LHRH-A3;采用2针注射,第一次注射剂量为(2～3)mg DOM+(4～6)μg LHRH-A3/kg体重,第二次注射剂量为(10～12)mg DOM+(20～24)μg LHRH-A3/kg体重。野生异齿裂腹鱼雌鱼群体卵巢发育不同步,即使同批催产的雌亲鱼其人工授精的适宜时间差异明显,在13.8～16℃温度条件下,其变化范围为34.5～81.43 h。产后亲鱼平均成活率56.5%。

The study was conducted on the technology of large scale artificial propagation and renewal cuhivation of mature wild broodstock of Schizothorax o＇connori after spawning from April fo June in 2010. Artificial fertilization of 108 females was conducted by dry-method, and the total 1040 thousand eggs were obtained, and 620 thousand larvae were hatched. There were 45 females naturally ovulating 468 thousand eggs without receiving hormone injection, and its average fertilization rate and hatching rate were lower than the induced spawning. 83 females were injected by external hormone to induce them to spawn, and the induced spawning rates ranged from 53.3% to 86.4% , and reproduced 570 thousand eggs, and some injected hormone females could spawn spontaneously. The combination of exterior hormones was the combination of DOM and LRHRH-A3, with one injection. The injection dosage was （ 10 - 12） mg DOM ＋ （20 - 24） μg LHRH-A3/kg female fish body weight, and with two injections, the first injection dosage was （2 -3 ）mg DOM ＋ （4 -6）μg LHRH-A3/kg female fish body weight, and the second was （ 10 - 12） mg DOM ＋ （20 - 24） μg LHRH-A3/kg female fish body weight. The ovary development of different wild S. o＇connori was outer-sync, and the optimum time range of artificial fertilization was 34. 5 - 81.43 h under the temperature of 13.8 - 16 ℃, and had distinct difference for the different females injected at the same time. The average survival rate of broodstocks after spawning was 56. 5%.