摘要
目的探讨甲状腺乳头状癌中钙磷脂结合蛋白Ⅱ(AnnexinⅡ)与钙结合蛋白S100A4的表达,分析其与甲状腺乳头状癌发生及淋巴结转移的关系以及二者的相关性。方法应用免疫组织化学SP法和原位杂交技术检测50例甲状腺乳头状癌组织、14例甲状腺腺瘤组织、26例结节性甲状腺肿组织和10例正常甲状腺组织中AnnexinⅡ、S100A4蛋白和AnnexinⅡmRNA的表达。结果甲状腺乳头状癌中AnnexinⅡ、S100A4蛋白和AnnexinⅡmRNA的阳性表达率分别为82.00%(41/50)、78.00%(39/50)和74.00%(37/50),均明显高于甲状腺腺瘤组织、结节性甲状腺肿组织及正常甲状腺组织(P<0.05);三者在癌组织中的表达与甲状腺乳头状癌淋巴结转移有关(P<0.05);AnnexinⅡ蛋白与S100A4蛋白的表达呈正相关性(r=0.380,P=0.025);AnnexinⅡ蛋白与AnnexinⅡmRNA的表达呈正相关性(r=0.434,P=0.008)。结论 AnnexinⅡ蛋白、AnnexinⅡmRNA及S100A4蛋白的高表达与甲状腺乳头状癌的发生和淋巴结转移密切相关,二者联合检测有助于鉴别甲状腺良恶性病变,并预测肿瘤的发展和预后。
Objective To investigate the expression of AnnexinⅡand S100A4 in papillary thyroid carcinoma and their correlations with the occurrence and lymphatic metastasis of papillary thyroid carcinoma.Methods The immunohistochemistry S-P and in situ hybridization technique was used to test the different expressions of AnnexinⅡ,S100A4 proteins and AnnexinⅡ mRNA in tissues of 50 papillary thyroid carcinoma,14 thyroid adenoma,26nodular goiter and 10 normal thyroid gland.Results The positive expression rates of AnnexinⅡ,S100A4 proteins and AnnexinⅡmRNA in papillary thyroid carcinoma were 82.00%（41/50）,78.00%（39/50） and 74.00%（37/50）,respectively.They were significantly higher than those in thyroid adenoma,nodular goiter and normal thyroid gland（P0.05）.The expression rates of AnnexinⅡ,S100A4 proteins and AnnexinⅡmRNA in papillary thyroid carcinoma were associated with lymph node metastasis（P0.05）.The expression of AnnexinⅡprotein was positive correlated with that of S100A4 protein（r=0.380,P=0.025）.The expression of AnnexinⅡprotein was positive correlated with that of AnnexinⅡmRNA（r=0.434,P=0.008）.Conclusion The over expressions of AnnexinⅡand S100A4 were closely related with the occurrence and lymphatic metastases of thyroid carcinoma.Combining detection of AnnexinⅡ and S100A4 would help to distinguish the benign or malignant disease of thyroid gland and predict tumor development and prognosis.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2011年第12期1397-1400,共4页
Cancer Research on Prevention and Treatment
