暗黑鳃金龟幼虫取食Bt后中肠细胞形态变化的初步研究

Preliminary analysis of the cellular morphological change in the midgut of Holotrichia parallela larvae fed with Bt

本研究利用透射电镜技术观察了暗黑鳃金龟幼虫取食Bt后不同时间其中肠细胞形态变化过程。结果表明:幼虫取食Bt后1～7d中肠不同的组织结构逐渐发生变化,第1天就出现了细胞膜溶解的现象,在第7天细胞膜完全溶解消失;其他器官出现如细胞核核膜溶解,细胞核消失,内质网消失等现象。经过中肠细胞一系列的病变,最终导致幼虫死亡。

Transmission electron microscope was used to observe the cellular morphological change in the midgut of the Holotrichia parallela Motschulsky fed with Bt. The results showed that when the larvae were fed with Bt from one to seven days, a series of change happened to different parts of the midgut. The cell membrane of the midgut cells dissolved on the first day, and disappeared on the seventh day. Other symptoms observed included： the karyotheca dissolved, the nucleolus and the endoplasmic reticulum disappeared. Through a series of changes in the midgut cells, the larvae finally died.