白腐真菌包埋技术研究

Entrapment Technique of White-rot Fungi

利用海藻酸钠,K-卡拉胶、明胶、PVA 4种载体对白腐真菌进行包埋,然后对包埋后的白腐真菌进行复活试验,通过对白腐真菌菌剂菌丝体生长的速度及浓密程度的比较来验证其复活率.同时综合保存效果、制备成本等来选择制备白腐真菌菌剂的优势载体.结果表明,白腐真菌在4种不同的载体中性质稳定,杂菌感染率减少.其中用PVA和海藻酸钠作为载体对白腐真菌进行包埋,制备而成的菌剂在本次实验中显示了最佳效果.但考虑到包埋交联过程中PVA放热以及试剂本身的毒性,因此在白腐真菌处理有机固体废物时,海藻酸钠小珠包埋法应是一种适合的选择.

This study ,using sodium alginate, K-carrageenan, gelatin, PVA as agents for the white-rot fungi preparation by entrapment technique, does the revivable test of white-rot fuugi which is entraped, through comparing the growth rate and the extent dense of White-rot fungi preparation mycelium to validate the revival of the rate. Atthe same time, it also the keeping effect and preparation costs to find out the best carrier agent for the white-rot fuugi preparation. The results show that white-rot fuugi which is in various Matrices by entrapment technique has stable character and fewer bacteria. Using PVA and sodium alginate as agents for preparation of white-rot fungi by entrapment technique in this experiment shows the best results. However, considering the release of heat and toxic reagents of PVA in the process of entrapment, therefore it uses the calcium alginate as agents for the white-rot fuugi preparation by entrapment technique which is a fit choice in the White-rot fungi treatment of organic solid wastes.