摘要
经等电点沉淀，得到的猪凝血酶粗品，用以壳聚糖多孔珠作为亲和吸附剂载体，以肝素为配基制成的吸附剂进行发离纯化，结果表明，通过一步亲和层析将猪凝血酶粗品提纯了１１．５倍，获得了比活力１５５３．７Ｕ／ｍｇ的凝血酶，收率高达７８．８％
A crude thrombin (ECe.4.21.5)was obtained from porcine plasma by means of precipitation at isoeletric point. The thrombin was purified by affinity chromatography on the heparin which was immobilized on chitosan porous beads. The purified thrombin had a specific activity 1553.7U/mg so the purification activitg rate was 11.5 fold and recovery rate 78.8%.re spectively.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2000年第1期31-33,共3页
Food Science
