信阳水牛PRL基因单核苷酸多态性与生长性状的相关性

Associations between single nucleotide polymorphisms in PRL gene and growth traits in Xinyang buffalo

以50头信阳水牛为材料,以GenBank公布的牛PRL基因序列(NC_007324)作为参照序列,设计PCR引物,对PRL基因的第2、4、5外显子进行变异检测,并将其多态性与部分生长性状进行相关分析。结果表明:试验牛群体中,每一外显子的PCR扩增产物经SSCP分析均出现不同带型;试验牛PRL基因第2、4、5外显子各存在1处SNP(2974G>A,7581 G>A,8558 G>-)。PRL基因第2外显子BB型个体坐骨端宽显著宽于AA型个体(P<0.05)。

Blood samples from fifty xinyang buffalos were extracted for genomic DNA and the primers for PRL gene （NC-007324） were designed to detect the polymorphisms in the second, fourth and fifth exons of this gene by PCR-SSCP and DNA sequencing, then the correlations between the polymorphisms and the growth traits of the buffalos were analyzed. The PCR-SSCP and DNA sequencing showed that in the tested buffalos each exon had shifted SSCP band and there was one SNP in each exon （2974G〉A, 7581G〉A, 8558 G〉-）. The correlation analysis showed that the hucklebone widths in the buffalos genotyped AA were significantly （P〈0.05） greater than those in the buffalos genotyped BB based on the second exon polymorphisms.