MTT法测定八种中草药体外抗肺癌细胞SPC-A1活性

Screening of activity of anti lung cancer cell lines SPC-A1 in vitro from 8 Chinese traditional medicine by MTT

[目的]建立一套高效、快速的体外中草药抗肺癌活性物质筛选模型。[方法]采用MTT法,对芸香草、地榆、甘草、柴胡、莪术、蜜蒙花、半边莲和旱芹等8种中草药体外抗肺癌细胞SPC-A1的活性进行检测。分析肿瘤细胞生长曲线,观察倒置显微镜下肺癌细胞在不同阶段的生长状况。[结果]在中草药活性物质作用下细胞尽管仍然贴壁,但出现萎缩及边缘清晰化,与生长状况良好的细胞形态区别明显。浓度为1.5mg/ml的莪术乙醇浸提液(ESCC)和甘草乙醇浸提液(ESCG)对体外培养的肺癌细胞SPC-A1有较强的抑制增殖作用,抑制率分别为:52.9%和51.6%,且抑制作用随着浓度的加大而加强。[结论]MTT法筛选可作为8种中草药抗肺癌细胞的一个基本筛选模型。

[objective] Build up the more higher effect and quickly means to screenting active substance in vitro through our test.[method] The inhibitory effect to SPC-A1 cells of Cymbopogon distans,Sanguisorba officinalis,Glycyrrhiza uralensis Bupleurum chinense,Curuma zedoaria,Buddleja officinalis,Lobelia chinensis and Apium graveolen and on the growth of two cancer cell line were tested with the MTT assay.Curve description and cell morphology was watched by inverted microscope [result] cells induced by active substancer cells would be condensing、clear brim,which were much difference from normal SPC-A1 cells thought they still adherent.results suggested that,ethanol soluble constituent of C.zedoaria（ESCC） and Ethanol soluble constituent of G.uralensis（ESCG） can inhibit proliferation of SPC-A1 cells at the concentration of 1.5mg/ml,and the inhibitory rate was： 52.9% and 51.6%,which increased with time in the range of the test.[conclusion] It＇s a good method to test anti-tumor activity by MTT.