高效液相色谱法测定调胃丹中厚朴酚、和厚朴酚含量

Determination of Magnolol and Honokiol in Tiaowei Pills by HPLC

目的建立调胃丹中厚朴酚、和厚朴酚的含量测定方法。方法采用Agilent Extend C18柱(250 mm×4.6 mm,5μm)为色谱柱,以乙腈-0.1%磷酸溶液(57∶43)为流动相,检测波长为294 nm,流速为1.0 mL/min。结果厚朴酚进样量在0.105 6～1.056 0μg范围内与峰面积具有良好线性关系,r=1.000 0;和厚朴酚进样量在0.044 9～0.448 8μg范围内与峰面积具有良好线性关系,r=1.000 0。厚朴酚的平均回收率为98.07%,RSD=1.14%(n=6);和厚朴酚的平均回收率为97.53%,RSD=0.60%(n=6)。结论所用方法结果简便、快速、准确,可用于调胃丹的质量控制。

Objective To establish a HPLC method for simultaneous determination of magnolol and honokiol in Tiaowei Pills. Methods The separation was performed on the Agilent Extend C18 column （250mm×4.6mm,5μm） with acetonitrile-0. 1% phosphoric acid （57：43） as the mobile phase. The UV detection wavelength was set at 294 nm and the flow rate was 1.0 mL/min. Results There was good linearity between the peak areas and concentrations in the range of 0. 105 6 - 1. 056 0 μg（ r = 1. 000 0） for magnoM and 0. 044 9 -0. 448 8 μg（ r = 1. 000 0） for honokiol respectively. The average recovery rates of magnolol and honokiol were 98.07%, RSD=1. 14% （n=6）and 97.53%, RSD =0. 60% （n =6）,respectively. Conclusion The method is rapid, simple, accurate and can be applied for the quality control of Tiaowei Pills.