鸭疫里默氏杆菌多价灭活苗研制的研究报告

Research report on development of multivalent inactivated vaccine of Duck Riemerella anatipestifer

用三个血清型的菌株,分别是JYL-1、JYL-2、JYL-7,用两种佐剂,分别是蜂胶和油乳剂共制备了两种多价菌苗,即JYL-1、JYL-2、JYL-7(1、2、7三个血清型)三个RA血清型混合多价蜂胶苗和油乳剂苗。试验结果表明,蜂胶复合佐剂多价苗具有产生免疫保护速度快,免疫持续时间长的优点,接种后第3天即产生部分免疫保护力,第120天时仍具有完全保护力;油乳剂多价苗产生保护力的速度较慢,接种后第10天时开始表现出部分免疫保护,其完全保护力也可持续到接种后第120天。免疫后13d时,免疫保护率可达90%左右。免疫后16d时保护率为100%。免疫后120d时,两种多价菌苗的免疫保护率均可达到100%,免疫后150～180d时,免疫保护率可达80%左右。统计学分析证明,菌苗的安全性良好,用3个免疫剂量的菌苗所做的安全试验表明无明显不良反应,菌苗在4℃保存一年,18～22℃室温保存4个月不影响菌苗的免疫效力。田间试验表明,用菌苗免疫鸭群后,可有效地使鸭群抵抗鸭疫里默氏杆菌的感染。

Two multivalent inactivated vaccines （multivalent propolis vaccine and oil emulsion vaccine） were prepared with mixed three serotype strains ofRiemerella anatipestifer （RA）： JYL-1, JYL-2, JYL-7, （1, 2, 7 three serotypes） using two adjuvants： propolis and oil emulsions, respectively. The results showed that polyvalent propolis-adjuvant inactivated vaccine had advantages such as a high speed production of immune protection and a long duration immunity. Partial protective immunity could be detected 3 days after inoculation, while complete protection were remained at the 120 days after inoculation. Polyvalent oil emulsion-adjuvant inactivated vaccine produced protection more slowly. Partial protective immunity could be detected 10 days after inoculation, and the complete protection were also remained at the 120 daysafter inoculation. 13 days after immunization, immunoprotection rate was up to about 90%. 16 days after immunization, the immunoprotection rate was 100%. 120 days after immunization, the immunoprotection rate of both two polyvalent vaccine could reach 100%, 150-180 days after immunization, immunoprotection rate was about 80%. Statistical analysis of safety tests showed that the safety of the vaccines was good, because immunization with triple doses of vaccine made no significant adverse reactions. It is not affect the immune efficiency of vaccines when storing them at 4℃ for one year, or at 18 - 22℃ （room temperature） for 4 months. Field tests showed that after immunization with the vaccines, the ducks could resist against the infection of Riemerella anatipestifer effectively.