肾性高血压大鼠模型制备方法的改进

IMPROVEMENT OF METHOD OF MAKING RENOVASCULAR HYPERTENSIVE MODEL IN RATS

目的:建立一种改良的普遍适用且成活率高的肾性高血压大鼠模型。方法:健康雄性SD大鼠32只,随机分为手术组(n=16)和假手术组(n=16)。采用缝合线和针灸银针对手术组大鼠造成单侧肾动脉狭窄,形成肾性高血压。假手术组仅游离肾动脉,不予缩窄。结果:肾动脉缩窄术后14d,手术组血压较术前明显增高(t=6.366,P<0.01),术后28d血压进一步升高,达到稳定的高水平(t=12.806,P<0.001);左肾动脉缩窄术后28d手术组血压显著高于假手术组(t=10.41,P<0.01),大鼠成活率75%(12/16),成活的大鼠全部形成高血压模型。结论:该动物模型可模拟人单侧肾动脉狭窄所致的高血压病变,其制作方法简便,可操作性强,成功率高,符合高血压实验研究的需要。

Objective：To establish an improved and universally applicable method of renovascular hypertensive animal model with higher survival rate.Methods：Healthful 32 male SD rats were randomly divided into operation-group and non-operation group.Renovascular hypertension in operation-group rats was induced by single renal artery narrow with suture and acupuncture needles.Renal artery in non-operation group rats was separated but not narrowed.Results：The blood pressure of renal artery narrow operation-group at 14 days after operation was higher than that before operation（t=6.366,P0.01）,and then the blood pressure go up to a steady and even higher level at 28 days after operation compared with blood pressure before operation（t=12.806,P0.001）.On the other hand,blood pressure in operation-group was increased obviously compared with non-operation group at 28 days after operation（t=10.41,P0.01） with high successful rate of 12/16.Conclusion：Our improved renovascular hypertensive model in rats could imitate hypertensive disease of human.The method is a simple technique,with strong feasibility,high successful rate,and suit needs of empirical study of hypertension.