农药高效氯氰菊酯对小鼠肾细胞氧化损伤的研究

Study on the oxidative damage of mouse kidney cells induced by pesticide beta-cypermethrin

为了研究农药高效氯氰菊酯对生物体的氧化损伤,以昆明小鼠为受试体,高效氯氰菊酯按10、20和40mg/kg 3个剂量水平,灌胃染毒小鼠7d,以肾匀浆测定ROS和GSH,以肾细胞测定DPC系数.实验结果表明:随着高效氯氰菊酯染毒剂量的升高,ROS和DPC系数逐渐上升,GSH含量逐渐降低,各指标呈一定的剂量-效应关系.染毒剂量≥20mg/kg时,ROS和DPC系数有显著差异(p<0.05),GSH有极显著差异(p<0.01),说明较高剂量的高效氯氰菊酯可造成小鼠肾脏的氧化损伤.

This study aims at the oxidative stress effects of pesticide beta-cypermethrin on organisms. Kunming mice were orally administrated with beta-cypermethrin for sev- en days at the concentration of 10, 20 and 40 mg/kg respectively , the contents of ROS and GSH in the kidney homogenate and DPC coefficients in the kidney cells were measured to indicate the oxidative damages. The experimental results showed that the con- tents of ROS and DPC coefficients increased gradually while GSH content decreased with the increasing exposure dose, all the biomarkers were in the exposure dose-response manner. When exposure dose was over 20 mg/kg, ROS content and DPC coefficient were significantly higher than the control group （p〈0.05） , and GSH contents had extremely significant differences （p〈0.01）. These experimental results demon- strate that heta-cypermethrin can increase the oxidative stress in mouse kidney at higher dose.