原生质体融合构建高效降解有机氯化物工程菌的研究

Construction of Engineering Microorganism Degrading Chlorinated Organic Compounds Efficiently by Protoplast Fusion

以供试菌Pseudomonas putida.和Psathyrella candolleana为出发菌株进行原生质体融合实验,筛选得到20株重组菌,并用于处理五氯酚(PCP)合成废水以检测重组菌的氯代酚降解性能。基于导数光谱建立测定废水中PCP含量的新方法,可快速鉴定各重组菌的氯代酚降解性能。结果显示,Xz6-1的PCP去除率为75.98%,其PCP降解能力较亲本Pseudomonas putida提高了21.71%。

Protoplast fusion was used to construct a new strain with an ability of degrading chlorophenols efficiently with Pseudomonas putida and Psathyrella candolleana. 20 recombinant strains were screened and used to degrade PCP synthetic wastewater in order to determine their capabilities of chlorophenol degradation. Also, a new method quantifying PCP was proposed based on derivative spectroscopy, wastewater, so chlorophenol biodegradability of recombinant strains which can indicate PCP content in synthetic could be measured rapidly. The results indicated that the PCP rerhoval rate of recombinant strain Xz6 - 1 was 75.98% and that of Pseudomonas putida was 54. 27% , which showed that the removal rate was improved by 21.71% by Xz 6 - 1 as compared with Pseudomonas putida.