新疆阿勒泰地区哈萨克族人群15个STR基因座的遗传多态性

Genetic polymorphism of 15 short tandem repeat(STR) loci in Kazakh population of Altay area of Xinjiang

目的调查阿勒泰地区哈萨克族无关个体的15个STR基因座(D8S1179、D21S11、D7S820、CSF1PO、D3S1358、TH01、D13S317、D16S539、D2S1338、D19S433、vWA、TPOX、D18S51、D5S818、FGA)多态性,研究其在法医学检验中的应用价值。方法用AmpFlSTR IdentifilerTM五色荧光标记系统对采集的280份血样进行15个基因座的扩增,用ABI3130XL全自动测序仪对扩增产物进行检测,用GeneMapper v3.3软件进行基因分型。结果 15个STR基因座在阿勒泰地区哈萨克族人群的累积个体识别率(TDP)大于0.999 999 999 9,累积非父排除率(CEP)达99.999%。结论该15个STR基因座可满足新疆阿勒泰地区哈萨克族人群法医学个体识别及亲权鉴定的需要。

Objective To investigate genetic polymorphism of 15 short tandem repeat（STR） loci（D8S1179,D21S11,D7S820,CSF1PO,D3S1358,TH01,D13S317,D16S539,D2S1338,D19S433,vWA,TPOX,D18S51,D5S818,FGA） in unrelated Kazakh individuals in Altay area and study its application value in forensic examination.Methods AmpFlSTR IdentifilerTM five-color fluorescent labeling system was employed to amplify 15 STR loci in 280 blood samples,ABI3130XL automatic sequencer was used to detect the amplification products and GeneMapper v3.3 software was applied for genotyping.Results The 15 STR loci in Kazakh population in Altay area showed an accumulated total discrimination power（TDP） exceeding 0.999 999 and the cumulative excluding probability of paternity（CEP） reaching 99.999%.Conclusion The 15 STR loci can meet the need of forensic individual identification and paternity identification in kazakh population in Altay area of Xinjiang.