外周血生存素的表达与结肠癌相关性研究

A study on the correlation of survivin expression in peripheral blood and colon cancer

目的探讨外周血生存素的表达与结肠癌的关系。方法以逆转录-聚合酶链反应-酶联免疫吸附测定(RT-PCR-ELISA)方法检测95例结肠癌患者外周血生存素mRNA的表达,比较其是否与肿瘤部位、浸润深度、分化程度、淋巴结转移和临床分期具有相关性。结果 35例(36.8%)结肠癌患者外周血生存素mRNA表达阳性。肿瘤高、中分化及低、未分化生存素mR-NA表达率分别为31.0%、45.0%。浸润深度达浆膜层者外周血生存素mRNA表达率(44.0%)高于侵及黏膜下层及肌层的表达率(20.0%),淋巴结有转移者外周血表达率为47.5%,高于无淋巴结转移的表达率(19.4%)。结论生存素mRNA在外周血的表达与肿瘤分化、浸润深度、淋巴结转移和临床分期均有明显相关性,但与肿瘤部位无明显相关。

Objective To explore the correlation of survivin expression in peripheral blood and colon cancer.Methods Reverse transcriptase-polymerase chain reaction and enzyme linked immunosorbent assay（RT-PCR-ELISA） was employed to detect the mRNA expression of survivin in peripheral blood of 95 patients with colon cancer.The correlations between the survivin mRNA expression and the tumor location,depth of invasion,differentiation degree,lymphatic metastasis or clinical stages were studied.Results 35（36.8%） patients with colon cancer showed positive survivin mRNA expression in peripheral blood.The expression rate of survivin in well and moderately differentiated tumor was 31.0% and that in poorly differentiated and undifferentiated tumor was 45.0%.The mRNA expression rate of survivin in peripheral blood of patients with serosal invasion（44.0%） was higher than that in patients with submucosa and muscular invasion（20.0%）,and the rate was 47.5% in patients with lymphatic metastasis,which was higher than that in patients without lymphatic metastasis（19.4%）.Conclusion Survivin mRNA expression in peripheral blood shows a significantly correlation with tumor differentiation,invasive depth,lymphatic metastasis and clinical stages,but not with tumor location.