优选野蔷薇中金丝桃苷的提取工艺

Study on the Extraction Process of Hyperoside from Rosa multiflora Thunb by Orthogonal Test

采用正交试验设计探讨超声辅助提取野蔷薇中金丝桃苷的最佳工艺。用HPLC法测定金丝桃苷含量。结果:最优提取条件为70%乙醇为提取溶剂,料液比为1:10,超声提取时间为40min,提取温度45℃。在最优因素水平组合条件下,金丝桃苷提取率为9.26μg/g(以干重计)。

Study the ultrasound-associated extraction of hyperin in rosa multiflora fruit objectively. The content of hyperin was determined by HPLC. The optimal ultrasound-associated extraction conditions were as follows： 70% ethanol extracting at 45 ℃ for 40 min and the ratio of material to solvent was 1 ： 10. Under these conditions, the extraction rate was 9.26 μg/g in dry weight.